Novel Method for Isolation of Adult Porcine Pancreatic Islets with Two-Stage Digestion Procedure
نویسندگان
چکیده
منابع مشابه
Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields.
During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardized and collagenase of controlled quality was used, we were unable to attain h...
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BACKGROUND A critical component of treating type I diabetes by transplantation is the availability of sufficient high-quality islets. Currently, islets can be obtained only by reliance on an expensive, inconsistent, and toxic enzyme digestion process. As an alternative, we hypothesize that cryobiologic techniques can be used for differential freeze destruction of the pancreas to release islets ...
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متن کاملFactors influencing isolation of functional pancreatic rat islets.
Yields and function of isolated islets vary considerably in spite of the introduction of new or improved methods for isolation. In most studies, these variations have been attributed to inadequacies of the applied collagenase preparations. However, when we retrospectively analyzed our rat islet isolations, we found large variations in yield and function in spite of application of identical coll...
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ژورنال
عنوان ژورنال: Cell Transplantation
سال: 1999
ISSN: 0963-6897,1555-3892
DOI: 10.1177/096368979900800408